Biochem/physiol Actions

Cell permeable: no

Reversible: no

Product does not compete with ATP.

Primary TargetChymotrypsin

General description

Sensitive fluorogenic substrate for the quantitative determination of chymotrypsin activity.

Sensitive fluorogenic substrate for the quantitative determination of chymotrypsin activity (excitation maximum: ~380 nm; emission maximum: ~460 nm).

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Irvine, G.B., et al. 1990. Anal. Biochem.185, 304.Graf, L., et al. 1987. Biochemistry26, 2616.Oshima, G. 1984. J. Biochem.95, 1131.Oshima, G. 1983. J. Biochem.94, 1615.

Packaging

25 mg in Glass bottle

Reconstitution

Following reconstitution aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 3 months at -20°C.

Sequence

Suc-Ala-Ala-Pro-Phe-AMC

Warning

Toxicity: Standard Handling (A)

assay	≥98% (HPLC)
color	white
fluorescence	λ_ex ~380 nm, λ_em ~460 nm
form	lyophilized solid
InChI key	IGKPQFYMWKQDQS-KCXKOMAXSA-N
InChI	1S/C34H39N5O9/c1-19-16-30(43)48-27-18-23(11-12-24(19)27)37-25(17-22-8-5-4-6-9-22)32(45)38-33(46)26-10-7-15-39(26)34(47)21(3)36-31(44)20(2)35-28(40)13-14-29(41)42/h4-6,8-9,11-12,16,18,20-21,25-26,37H,7,10,13-15,17H2,1-3H3,(H,35,40)(H,36,44)(H,41,42)(H,38,45,46)/t20-,21-,25-,26-/m0/s1
manufacturer/tradename	Calbiochem^®
Quality Level	100
shipped in	ambient
solubility	acetonitrile: water (1:1): soluble, DMSO: soluble
storage condition	desiccated, protect from light, OK to freeze
storage temp.	−20°C

Safety Data Sheet for 230914-25MG

Cas Number

88467-45-2

This product has met the following criteria to qualify for the following awards:

Chymotrypsin Substrate II; Fluorogenic

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